recombinant jagged1 protein  (MedChemExpress)

Journal: BDJ Open

Article Title: MicroRNA expression in JAG1/Notch-activated periodontal ligament stem cells

doi: 10.1038/s41405-024-00232-5

Figure Lengend Snippet: Representative histogram indicating expression of MSCs markers as evaluated by flow cytometry ( a ). Multilineage differentiation of the osteo/odontogenic and adipogenic lineages examined by alizarin red s staining and oil red o staining ( b ). Cells were seeded on Jagged1 immobilized surface and maintained in osteo/odontogenic medium. Cells on hFc-immobilized surfaces were used as controls. Osteo/odontogenic differentiation was evaluated in vitro using alizarin red s staining ( c ).

Article Snippet: Briefly, recombinant protein G (Sigma-Aldrich, USA) was incubated in a tissue culture plate at a concentration of 50 μg/mL for 16 h. The surface was then incubated with 10 mg/mL of BSA (Sigma-Aldrich, USA) for 2 h followed by incubation with Jagged1/Fc (R&D systems, USA) for 2 h. An equal volume of human IgG, the Fc fragment (Sigma-Aldrich, USA), was used as a control.

Techniques: Expressing, Flow Cytometry, Staining, In Vitro

Journal: BDJ Open

Article Title: MicroRNA expression in JAG1/Notch-activated periodontal ligament stem cells

doi: 10.1038/s41405-024-00232-5

Figure Lengend Snippet: Heatmap of significantly alter miRNAs in Jagged1-treated PDLSCs ( a ). Venn diagram of predicted target genes of the significantly altered miRNAs ( b ).

Article Snippet: Briefly, recombinant protein G (Sigma-Aldrich, USA) was incubated in a tissue culture plate at a concentration of 50 μg/mL for 16 h. The surface was then incubated with 10 mg/mL of BSA (Sigma-Aldrich, USA) for 2 h followed by incubation with Jagged1/Fc (R&D systems, USA) for 2 h. An equal volume of human IgG, the Fc fragment (Sigma-Aldrich, USA), was used as a control.

Techniques: